anti gr 1 rat monoclonal antibody  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: Blocking P2Y2 purinergic receptor prevents the development of lipopolysaccharide-induced acute respiratory distress syndrome

doi: 10.3389/fimmu.2023.1310098

Figure Lengend Snippet: PMN migration into the interstitium and BALF. PMN migration in the various lung compartments (interstitial, alveolar space) was assessed followed by i.t. injection of Alexa 633-labeled rat anti-mouse GR-1 (10 µg) antibody in control C57BL/6 wild type mice and (A) Suramin 100 µM- and (B) PPADS 100 µM-treated mice over 48 h. Statistical analysis was based on Bonferroni post-test. Values are given as mean ± SEM. n = 4 mice in each group. *p < 0.05, **p < 0.01 versus antagonist LPS.

Article Snippet: Briefly, mouse BALF cells were incubated with unlabeled rat anti-mouse CD16/CD32 antibody (Mouse BD Fc Block, BD Biosciences, Germany) to block Fc receptors and stained for 20 minutes with APC-conjugated Armenian hamster monoclonal anti-mouse CD11c (Thermo Scientific, Germany), FITC conjugated rat monoclonal anti-mouse Ly-6G (Gr-1) (Thermo Scientific, Germany), PE-Cy7-conjugated rat monoclonal anti-mouse CD3e (Thermo Scientific, Germany), PE-Cy7-conjugated rat monoclonal anti- B220 (Thermo Scientific, Germany) and PE-conjugated monoclonal anti-mouse F4/80 (Miltenyi Biotec, Germany), in PBS containing 0.5% BSA and 0.01% sodium azide.

Techniques: Migration, Injection, Labeling

Journal: Frontiers in Immunology

Article Title: Blocking P2Y2 purinergic receptor prevents the development of lipopolysaccharide-induced acute respiratory distress syndrome

doi: 10.3389/fimmu.2023.1310098

Figure Lengend Snippet:

Article Snippet: Briefly, mouse BALF cells were incubated with unlabeled rat anti-mouse CD16/CD32 antibody (Mouse BD Fc Block, BD Biosciences, Germany) to block Fc receptors and stained for 20 minutes with APC-conjugated Armenian hamster monoclonal anti-mouse CD11c (Thermo Scientific, Germany), FITC conjugated rat monoclonal anti-mouse Ly-6G (Gr-1) (Thermo Scientific, Germany), PE-Cy7-conjugated rat monoclonal anti-mouse CD3e (Thermo Scientific, Germany), PE-Cy7-conjugated rat monoclonal anti- B220 (Thermo Scientific, Germany) and PE-conjugated monoclonal anti-mouse F4/80 (Miltenyi Biotec, Germany), in PBS containing 0.5% BSA and 0.01% sodium azide.

Techniques: Enzyme-linked Immunosorbent Assay, Fluorescence, FACS, Knock-Out, Saline, Real-time Polymerase Chain Reaction